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KMID : 0545119990090040436
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 4 p.436 ~ p.442
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Symmetry Region at Beginning of Transcript Inhibits Expression of Escherichia coli aeg-46.5 Operon
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Lee, Seung Hwa
Lee, Sang Ho/Sung, Ha Chin/Kim, Joon/Choe, Mu Hyeon
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Abstract
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The aeg-46.5 operon of Escherichia coli is induced by nitrate and anaerobic conditions. Positive regulators Fnr and NarP, and a negative regulator NarL control the expression of the aeg-46.5. It has two symmetry regions [6], one of which is located between +37 and +56 bp from the 5´end of the anaerobic transcription initiation site. In this study, mutagenized symmetry regions were transferred from plasmid to chromosome by homologous recombination to evaluate the mutation as a single copy in the fnr, narL, narP, and narL-narP double mutant background. The expressions of the aeg-46.5 operon with these mutations indicated that the control was not through the possible stem-loop structure. Whether there is a protein that mediates this control remains to be seen. The results from the narL-narP double mutant indicated that the anaerobic Fnr induction was independent of NarL repression.
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